Composite

Part:BBa_K531011:Design

Designed by: Alexander Aaring   Group: iGEM11_Grinnell   (2011-09-28)


Constitutive promoter and RBS BBa_K081005 + Caulobacter optimized esp + C-terminal sec


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 292
    Illegal XhoI site found at 862
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1117
    Illegal AgeI site found at 1240
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Optimized esp does not have a stop codon to ensure expression of the fused secretion tag.


Source

BBa_081005 was submitted to the registry by 2008 iGEM Team UNIV_Pavia, codon optimized esp was synthesized by Integrated DNA Technologies, rsaA C-terminal secretion tag was copied from Caulobacter lab strain CB15N.

References