Composite
Part:BBa_K531011:Design
Designed by: Alexander Aaring Group: iGEM11_Grinnell (2011-09-28)
Constitutive promoter and RBS BBa_K081005 + Caulobacter optimized esp + C-terminal sec
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 292
Illegal XhoI site found at 862 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1117
Illegal AgeI site found at 1240 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Optimized esp does not have a stop codon to ensure expression of the fused secretion tag.
Source
BBa_081005 was submitted to the registry by 2008 iGEM Team UNIV_Pavia, codon optimized esp was synthesized by Integrated DNA Technologies, rsaA C-terminal secretion tag was copied from Caulobacter lab strain CB15N.